Antioxidant and anticancer potential of methanolic leaf. Evaluation of some plant extracts for standardization and. Mcf7 and mdamb231 breast cancer cell lines were used to study the cytotoxic effect of different concentrations 20200 gml of ethanol and aqueous extracts of. In vitro antioxidant and anticancer activity of leea. Cell death caused by the whole plant extracts is via apoptosis.
To demonstrate the potential biological activity of alcoholic extracts, we carried out a screening of the extracts by measuring their in vitro antiproliferative properties against several common human cancer cell lines. Invitro anticancer activity of few plant extracts against. In recent years owing to the fear of side effects people prefer to use natural plant products for cancer treatment. Research article anticancer activity, antioxidant activity, and phenolic and flavonoids content of wild tragopogon porrifolius plant extracts fuadalrimawi, 1 suzirishmawi, 2 sharehanh. Antioxidant, anticancer activity and phytochemical. Petroleum ether 4060 c, chloroform, ethyl acetate and methanol 80% extracts of c. Anticancer activities of plant extracts of gymnacranthera. Pdf in vitro anticancer and cytotoxic activities of some. The anticancer activity of the different extracts of fruits of piper longum on human lung epithelial adenocarcinoma cell line hcc827 has been assessed in vitro using 34,5dimethylthiazol2yl2,5diphenyl tetrazolium bromide mtt assay. As previously mentioned polyphenols can inhibit or alter the regulation of proteins and other agents which may be contributing to the survival of cancer cells. The dpph radical scavenging assay exhibited higher antioxidant activity ic50 9. Cell lines derived from primary and recurrent brain tumors from the same patient and cells. In order to meet this demand, it is important to study sources of fibers and antioxidants.
The anticancer activities of vernonia amygdalina extract in. However, current research about the mechanisms of the anticancer potential of va. The evaluation of the anticancer activity of plant extracts is essential for safe treatment. Evaluating medicinal plants for anticancer activity elisha solowey, 1 michal lichtenstein, 1 sarah sallon, 2 helena paavilainen, 2 elaine solowey, 3 and haya lorberboumgalski 1 1 department of biochemistry and molecular biology, institute for medical research israelcanada imric, hebrew university hadassah medical school, 91120.
Antimicrobial activity of medicinal plant extracts and their synergistic effect on some selected pathogens desalegn amenu college of natural and computational science, biology department, wollega university, p. The nanoparticle synthesized was subjected for its anticancer activity against ht 29 colon cancer cell line and found ic50 value of 26. Since all these plants were showed some anticancer activity with different extracts and in different cell lines as per the references and based on ayurvedic literature, these plants were chosen for the present study. Preparations of the plant are commonly used across nigeria for the treatment of inflammatory conditions. Anticancer activity of the same fraction was determined using mts in vitro assay. Medicinal plants of asian origin having anticancer potential. Antioxidant and anticancer activity of spirulina platensis. Crude extracts were prepared from the leaves of clidemia hirta using ethanol, petroleum ether and chloroform. In vitro and in vivo anticancer activity of root extracts. Plant derived compounds have demonstrated properties to inhibit cancer cell activity such as inhibiting proliferation of cancer cells and inducing apoptotic cell death. We assessed the ability of an ethanolic extract of s.
Copper nanoparticles as therapeutic anticancer agents. Selected plants have been explored for biological activity and further investigations into anticancer activity of the plants showing promising activity, must be undertaken. The whole plant extract caused the cells to apoptose and sensitized them to common cytotoxic drugs. Over a hundred essential oils from more than twenty plant families have been tested on more than twenty types of cancers in last past ten years. Antioxidant, anticancer activity and phytochemical analysis. Anticancer potential of medicinal plants and their. The antioxidant activity of spirulina water extracts was measured based on the scavenging activity of the stable 1, 1diphenyl 2picrylhyorazyl dpph free radical according to the method described by choi et al. Screening of different plant components in search of anti cancer drugs is one of the main research activities throughout the world.
Thus, we have made an attempt to use this herbal plant to check the efficacy against prostate cancer cell lines. Pdf in vitro and in vivo methods for anticancer activity. In vitro antioxidant and anticancer activity of leea indica. Characterization techniques several techniques are employed for the. Research article evaluating medicinal plants for anticancer. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Khalid, 2 ismailwarad, 3 andzaidounsalah 2 chemistry department,facultyof science and technology,alqudsuniversity, p. Anacardiaceae is believed to have activity against gastrointestinal, neurological and endocrine diseases. For prevention and treatment of several diseases plant and plant extracts have been widely used all over the world. Previous research conducted on compound 2 against pc3 and mcf7 cell lines reported ic 50 values of 3. Plantderived anticancer agents in clinical use figure 1. Therefore, it might be beneficial as a medicinal plant for alternative novel anticancer drugs and nutraceutical products. Anticancer activity of extracts derived from the mature roots. We tested a variety of cancer cell lines originating from various human tumors including hematopoietic tumors such as t cell lymphoma and leukemia, as well as solid tumors such as colon, renal.
Anticancer activity, antioxidant activity, and phenolic and. Whole plant extracts ethanol extracted were prepared, coded as plant extract numbers 117, and tested for their potential as anticancer agents. We tested a variety of cancer cell lines originating from various human tumors including hematopoietic tumors such as t cell lymphoma and leukemia, as well as solid tumors such as colon, renal, breast, bladder. The complete inhibition activity of sarang semut extracts to the growth of both hela cells and mcmb2 cells was shown in both table 1 and 2. In vitro anticancer activity and cytotoxicity screening of. In vivo anticancer activity of basella alba leaf and seed extracts. Evaluating medicinal plants for anticancer activity. The extracts were analyzed for their total phenolic content tpc, total flavonoid content tfc, and antioxidant activity.
Journal of chemical and pharmaceutical research, 2018, 10. In conclusion, the present study demonstrated that the herbal extracts of a. Anticancer activity of ethanol extracts of polygala. Pharmacological evaluation of the anticancer activity of. Evidences have been accumulated since ages to demonstrate promising potential of medicinal plants used in various traditional, complementary, and alternative systems especially for cancer treatment. Aug 16, 2006 flavonoidrich extracts from the mature roots of scutellaria baicalensis have been shown to exhibit antiproliferative effects on various cancer cell lines. Breast cancer is currently the leading cause of cancerrelated deaths among women globally. To evaluate anticancer activity of the barleria grandiflora acanthacae leaf. The anticancer activity of ag nps was highly supported by the works of prabhu 21. The fraction showed significant antireverse transcriptase activity in the enzymebased direct binding assay which was as good as that of the standard synthetic inhibitor. The effect of plant extracts as anti cancer was widely.
Medicinal plants have a special place in the management of cancer. Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method. Evaluation of the cytotoxic activity of extracts from. Ethanolic extracts of dried plant was obtained by soxhlet extraction method and aqueous extracts was obtained by maceration method using ethanolic and aqueous solvents respectively. Anticancer activity of natural compounds from plant and marine environment. However, current research about the mechanisms of the anti. Anticancer activity of whole plant ofamaranthus tricolor. The anticancer activity of the crude methanolic leaf extract of m. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent. In vitro studies of the anticancer action of tectaria. In vitro anticancer activity of the crude extract and two. Anticancer activity, antioxidant activity, and phenolic. The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay.
Box, 395, ethiopia abstract antibiotics provide the main basis for the therapy of microbial bacterial and fungal infections. Samples of tragopogon porrifolius plant grown wild in palestine were extracted with different solvents. Antioxidant and anticancer activities of moringa oleifera leaves. To evaluate the anticancer and antioxidant activity of medicinal plant clidemia hirta extracted in different solvents. Anti cancer activity of four selected mangrove plants bruguiera gymnorrhiza, aegiceras corniculatum, aegialitis rotundifolia and lumnitzera racemosa against hepg2 cell line was studied. The obtained extracts were dried and used for investigation of anticancer activity. Forty eight extracts from 38 medicinal plants distributed into 19 different plant families were evaluated for cytotoxic activity on mammalian cells llcmk2 cells as presented in table 1.
The nature of cytotoxic activity is found to be apoptotic rather than necrosis by hoechst test. The present study investigates comparative in vitro antioxidant activities via different assays, anticancer activity via. Only anecdotal publications have been devoted to the anticancer activ. Moghe4 1department of microbiology, bhavans college, andheri w, mumbai. Pdf anticancer activity of morus nigra leaves extract. Vinca r osea alkaloids, vinblastine and vincristine, are one of the most potent anticancer drugs known. In vitro screening for cytotoxic activity of herbal extracts. Anticancer activity of ethanolic and aqueous extracts was investigated by using mcf7 and mdamb231 breast cancer cell lines. Thus, plant extracts 5, 10, and 11 exhibited time and concentrationdependent anticancer activity while having no effect on primary cell cultures from healthy humans. In western africa ethnomedicine, lannea barteri oliv. Pdf evaluating medicinal plants for anticancer activity. In vitro anti cancer activity of mangrove plant extracts. Pdf anticancer activity of ethanol extracts of polygala.
The potency of plant extracts to inhibit the cancerous growth was recordedin terms of decrease in. Sansevieria liberica gerome and labroy agavaceae is a perennial plant widely distributed in tropical africa. Vinca alkaloids and cytotoxic podophyllotoxins were discovered in the 1950s as first anticancer agents from plants. Anticancer activity of the plant extracts were also tested on hos and khos osteosarcoma cell lines. It was found that both tpc and aa are highest for plant extracted with 80% ethanol, followed by water, and finally with 100% ethanol. Some plant extracts exhibited antiproliferative activity toward all the cancer cell lines. Anticancer activity of essential oils and their chemical. The study was facilitated by collecting the plant sample and subjected to crude extraction.
All extracts in concentration 100 jig ml1 showed anticancer activity in pc3 and hela and the highest percentage of growth inhibition obtained from stems methanol extracts on hela 91. It is estimated that plantderived compounds in one or the other way. Anticancer activity of natural compounds from plant and. Camptothecin was introduced to clinical trials by the national cancer. The anticancer activities of vernonia amygdalina extract. Although morus alba methanol extract showed the most potent antiproliferative activity and could be investigated for finding pure anticancer compounds, but ic50 values 100 of mentioned plant extracts after 48, 96 and 144 h incubation period. Flavonoidrich extracts from the mature roots of scutellaria baicalensis have been shown to exhibit antiproliferative effects on various cancer cell lines. Antioxidant, anticancer activity, spirulina platensis, water extracts introduction the food industry has a challenge to produce low cost, nutritive and convenient foods. In vitro anticancer and cytotoxic activities of some plant. Evaluation of the anticancer activity of various extracts. Plant extract 5 urtica membranacea showed particularly strong anticancer capabilities since it inhibited actual tumor progression in a. Concentrationresponse curves for the antiproliferative. The antitumor activity of 50% ethanol extract 100 mgkg of a. Anticancer activity of essential oils and other extracts from.
Antimicrobial activity of medicinal plant extracts and. It enables identification of the intrinsic toxicity of the plant and the effects of acute overdose. In vitro anti cancer activity of mangrove plant extracts at various concentrations 10 to 200ugml was studied against the chosen cell line using mtt assay 34, 5dimethylthiazol2yl2, 5diphenyltetrazolium bromide. Anticancer activity using mcf7 human mammary gland breast adenocarcinoma cell lines december, 2011 drug invention today, 2011, 312, 290296 291 this division is similar to the above mentioned cancer division in humans. The killing activity was specific toward tumor cells, as the plant extracts had no effect on primary cultures of healthy human cells.
Anticancer activity of aloe vera and calligonum comosum. Treatment with the leaf extracts also exerted an apoptosisinducing effect in the cancer cells. Antioxidant and anticancer activities of moringa oleifera. Evaluation of antimitotic activity of solanum torvum using. According to recent studies conducted by the world health organization who, about 80% of the worlds population relies on traditional medicine. National cancer institute collected about 35,000 plant samples from 20 countries and has screened around 114,000 extracts for anticancer activity. Issn 23203862 evaluation of anticancer activity of stem. Comparative phytochemical investigation, antioxidant and. Anticancer potential of plants and natural products.
Anticancer activity test for extracts of sarang semut plant. However, as described in sections 3 to 5, a number of agents are in preclinical development. Research article anticancer activity, antioxidant activity. The aim of our study was to evaluate the effect of in vitro anticancer and cytotoxic activity of the methanolic extracts of 14 medicinal plants, 8 of which are endemic species in anatolia, against the human hela cervical cancer cell line and to compare to the normal african green monkey kidney epithelial cell line vero using the mtt. Notably, medicinal plant extracts may be a potential source for treatments of breast cancer. The results revealed that the polarity of the extraction solvent affects the tpc, tfc, and aa. Moringa oleifera, antioxidant activity, quinone reductase, antiproliferation, cancer. Plants are screened for treatment of many ailments including cancer because they possess certain potential constituents which are effective for treatment. This result may come from flavonoid and tannin compound contained in tested extracts. Herbal products are worldwide accepted as a source of complementary and alternative medicine in 14 various diseases especially in cancer 15. Tragopogon porrifolius, commonly referred to as white salsify, is an edible herb used in folk medicine to treat cancer.
Anti reverse transcriptase and anticancer activity of stem. Apoptosis is evaded and never induced in cancer cells and angiogenesis is sustained within the tumour tissue 12 allowing survival of cancer cells. The uses, mechanisms of action, and doses of natural compounds from plants and. It could also provide an indication of possible cytotoxic properties of the tested plant extracts. The results revealed that the occurrence of morphologically altered cells was greatest in the h 2 o 2treated group. Pdf plants are screened for treatment of many ailments including cancer because they possess certain potential constituents which are effective for. Plant extract 5 urtica membranacea showed particularly strong anticancer capabilities since it inhibited actual tumor progression in a breast adenocarcinoma mouse model. The mtt assay results were used to evaluate the anticancerous activity of the extract. Oct 01, 2015 other studies have looked at flavonoid extracts from fern species and found that even in low concentrations they still demonstrate high percentage of anticancer activity 23.
Anticancer activity of zea mays leaf extracts on oxidative. Based on the fact that herbal medicine is a strong component of integrative medicine, this study was conducted to evaluate the anticancer activity of root extracts. Anticancer activity of extracts derived from the mature. Study of antioxidant and anticancer activity of natural. Anticancer activity of carica papaya extracts in and. The data suggest that tc methanolic extract exhibited potent cytotoxic activity with an ic50 value less than 100.
The potential anticancer activity of the crude extract of ball moss and isolated dicinnamates are presented in table 1. The dpph radical scavenging activity is one of the most widely used methods for screening the antioxidant activity of plant extracts. Anticancer activity is the effect of natural and synthetic or biological and chemical agents to reverse, suppress or prevent carcinogenic progression. Aromatic plants have a long and significant history in the traditional medicine of many countries. Previous studies on this plant have revealed antimicrobial, anticholinestrase, anticonvulsant, antioxidant and antiinflammatory activities. Currently more than 3000 plants around world have been reported to possess anti cancer properties. Cytotoxicity evaluation of methanol extracts of some. Vernonia amygdalina va is a woody shrub reported to have not only diverse therapeutic effects but also anticancer properties. The present study confirms that the crude leaf extract of m. Newman encyclopedia of life support systems eolss reached the stage of general use. Pdf anticancer activity of morus nigra leaves extract researchgate. The data showed that the extracts of sarang semut plant have capability to inhibit the growth of hela and mcmb2 cells.
During the present investigation, crude methanol extracts of selected mangrove plants were prepared using soxhlet apparatus. We tested a variety of cancer cell lines originating from various human tumors including hematopoietic tumors such as t cell lymphoma and leukemia, as well as solid tumors such as colon, renal, breast, bladder, endometrial, and others table 1. Anticancerous activity of green tea and citrus limetta fig. Anticancer activity of uncommon medicinal plants from the. Nov, 2014 plant extracts 5, 10, and 11 were tested also on primary lymphocytes from healthy control donors and showed no killing activity on these cells figure 2c. Pharmacologically active flavonoids from the anticancer. In recent years, medicinal plants have attracted huge attention due to their diverse range of biological and therapeutic properties. To date, metaanalyses have primarily focused on the functions of dietary. Antioxidant and in vitro cytotoxic activity of extracts of.
Nowadays, there is an increasing interest in investigating the biological properties of aromatic plant extracts mainly due to their diversity, high availability, and low toxicity. Hence, these meristamatic cells can be used for preliminary screening of drugs with anticancer activity. Cell lines derived from primary and recurrent brain tumors from the same patient and cells selected for. The effects of plant leaves extract on cancer cells was studied. In vitro antioxidant and anticancer activity of leea indica leaf extracts. Both extracts could have cytotoxic and genotoxic activity. Correspondence mohan vr petchiparai, the whole plant of department of biomedical science, noorul islam center for higher education, kumaracoil, tamil nadu, india invitro anticancer activity of few plant extracts against mcf7, mdamb468 and mdamb231 cell lines rajesh a, doss a, tresina ps and mohan vr.
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